Evaluation of Relative Yeast Cell Surface Hydrophobicity Measured by Flow Cytometry
نویسندگان
چکیده
منابع مشابه
Evaluation of relative yeast cell surface hydrophobicity measured by flow cytometry.
OBJECTIVE To develop an efficient method for evaluating cell surface hydrophobicity and to apply the method to demonstrate the effects of fungal growth conditions on cell surface properties. METHODS Yeast isolates were suspended in phosphate-buffered saline and mixed with deep blue-dyed polystyrene microspheres. Flow cytometry was used to detect the degree of microsphere binding to yeast cell...
متن کاملCell-Cycle Analysis of Fission Yeast Cells by Flow Cytometry
The cell cycle of the fission yeast, Schizosaccharomyces pombe, does not easily lend itself to analysis by flow cytometry, mainly because cells in G(1) and G(2) phase contain the same amount of DNA. This occurs because fission yeast cells under standard growth conditions do not complete cytokinesis until after G(1) phase. We have devised a flow cytometric method exploiting the fact that cells i...
متن کاملEvaluation of the platelet surface by fluorescence flow cytometry.
Flow cytometry has significantly contributed to cell biology, especially with use of tagged monoclonal antibodies. Discovery of specific platelet surface membrane receptors has helped to elucidate the pathogenesis of several platelet related disorders. Concomitantly, monoclonal antibodies to specific receptors have also been developed. Using fluorescent antibody techniques, small populations of...
متن کاملSurface modifying substances that reduce apparent yeast cell hydrophobicity.
OBJECTIVE To determine whether several topical compounds and other chemical entities are able to diminish the surface hydrophobicity of yeast cells. METHOD Hydrophobicity of yeast cells was determined by binding styrene microspheres to the surface of untreated yeast or yeast pre-incubated with various substances with potential for cell surface modification. The degree of microsphere adherence...
متن کاملRelationship Between Cell Surface Hydrophobicity and Degradation of Hexadecane
Some properties of compounds in degrading bacteria are required for biodegradation of contaminants to higher performance. Those strains which have a high percentage of these features are more effective at biodegradation. The present experiments were designed to measure these parameters. In this study, measurement of cell surface hydrophobic-degrading bacteria was designed which oil was separate...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Infectious Diseases in Obstetrics and Gynecology
سال: 2005
ISSN: 1064-7449,1098-0997
DOI: 10.1155/2005/739101